Journal: Frontiers in Microbiology
Article Title: Genotypic and Phenotypic Characterization of Novel Sequence Types of Carbapenem-Resistant Acinetobacter baumannii , With Heterogeneous Resistance Determinants and Targeted Variations in Efflux Operons
doi: 10.3389/fmicb.2021.738371
Figure Lengend Snippet: Comparative profiles of Biolog Phenotype MicroArray analysis of A. baumannii DR1, DR2, and AB067 strains. Phenotypic microarray analysis of A. baumannii DR1, DR2, and AB067 strains to determine metabolic utilization profiles using PM 09 to PM20 plates. Kinetic growth response curve for A. baumannii DR1 and DR2 (A) and A. baumannii DR1 and AB067 (B) strains in PM09 to PM20 plates (top panel left as PM09 to right bottom end panel as PM20). Growth kinetics of A. baumannii DR2 shown in green, compared with DR1 strain in red. Yellow indicates equivalent growth response or overlapping of DR1 and DR2 kinetic curves. Red kinetic response curves indicate a stronger response by DR1 strain, whereas green indicates a stronger growth response by DR2 strain. Heat maps of selected phenotype microarray profiles of A. baumannii DR1, DR2, and AB067 strains for antimicrobial compounds (C) , toxic (D) , and miscellaneous compounds (E) . Each dot represents Omnilog values representing all time points of bacterial growth. Omnilog values of 100 or more were considered for phenotype analysis. Gradient heat maps were generated among strains for each condition using Omnilog values in Morpheus online tool ( https://software.broadinstitute.org/morpheus ), which displayed blue color for relative lower growth, and red color represents high growth phenotype.
Article Snippet: Subsequently diluted in Biolog fluid containing dye A (Biolog, Inc.), further 100 μl of diluted cell suspension was added to each well of the Biolog microplates and incubated in Omnilog automatic plate reader (Biolog, Inc.) for 48 h at 37°C.
Techniques: Microarray, Generated, Software
